Pharmacological inhibition of STING signaling attenu-ates MPTP-induced neuroinflammation and neurode-generation in experimental models of Parkinson's disease / 中国药理学与毒理学杂志

OBJECTIVE To investigate the effects of pharmacological inhibition of STING by C-176,a STING selective inhibitor,in experimental model of Parkinson's disease.METHODS The acute and sub-acute mice mod-els of Parkinson's disease(PD)were established by in-traperitoneal injection of 1-methyl-4-(2′-methylphenyl)-1,2,3,6-tetrahydrophine(MPTP).The selective STING inhibitor C-176 was administered by intraperitoneal injec-tion.The potential neuroprotective effects of C-176 were evaluated by behavioral test,tyrosine hydroxylase(TH)immunostaining,Nissl staining,Western blotting,qPCR and immunofluorescence.For in vitro study,the effects of C-176 on LPS/MPP+-induced inflammatory responses in BV2 microglial cells were determined by real time RT-PCR and Western blotting analysis.RESULTS Our study revealed that C-176 significantly inhibited STING signaling activation,ameliorated MPTP-induced dopami-nergic neurotoxicity,motor deficit and associated neuroin-flammation.Furthermore,pharmacological inhibition of STING in BV2 microglia treated with LPS/MPP+ exhibited decreased inflammatory responses.More importantly,C176 also reduced NLRP3 inflammasome activation both in vitro and in vivo.CONCLUSION The results of our study suggest that pharmacologic inhibition of STING protects against neuroinflammation that may act at least in part through suppressing NLRP3 inflammasome acti-vation and thus ameliorated dopaminergic neurodegener-ation.STING signaling may holds great promise for the development of new treatment strategy for PD as an effective therapeutic target.

Emergency Management of Medical Equipment in Designated Hospitals for Public Health Emergencies / 中国医疗器械杂志

Medical supply is a key resource for responding to public health emergencies and maintaining people's lives and health. As the medical equipment management department, the medical devices department is mainly responsible for the procurement, supply, technical support, management and coordination of medical equipment and medical consumables, playing an important role in epidemic prevention and control. Through the analysis of the expansion cases of designated hospitals, the experience of emergency management of medical equipment has been accumulated, which has strong practicability and replicability.

Investigation on Compatibility Stability of Calcium Gluconate Injection with Different Solvents / 中国药房

OBJECTIVE：To investigate the compatibility stability of Calcium gluconate injection with different solvents within 24 h，and to provide reference for clinical drug use. METHODS ：10％ Calcium gluconate injection was mixed with 0.9％ Sodium chloride injection and 5％ Glucose injection in the proportion of 10∶100，30∶100 and 50∶100（v/v）as trial group ，and mixed with 10％ Glucose injection in the same proportion as control group. The water was mixed with 0.9％ Sodium chloride injection ，5％ Glucose injection and 10％ Glucose injection in the same proportion as the blank control group. The appearance of the infusion in the trial group and the control group was observed within 24 h after preparation. pH value and the number of insoluble particles were detected and compared with the blank control group. The content of 5-hydroxymethylfurfural and the change of UV absorption spectrum were determined by UV spectrophotometry. RESULTS ：Compared with the blank control group infusion at the same time ， the infusion of trial group and the control group were colorless ，clear solution ，no visible foreign body ，and the pH value of the infusion of trial group and the control group had no significant change within 24 h. Within 24 h after preparation ，the number of insoluble particles ≥10 and ≥25 μm in 3 groups fluctuated but met the pharmacopoeia standard ；the number of insoluble particles with small particle size （5-10 μm）increased slightly with time ，but there was no significant difference between trial group ，control group and blank control group. The linear range of 5-HMF was 0.149-4.751 μg/mL（R2＝0.999 9）；the limit of quantitation was 0.013 μg/mL；RSDs of precision ，repeatability and stability tests （24 h）were less than 2％；average recovery was 105.23％ （RSD＝1.08％，n＝9）. The content of 5-HMF and the UV absorption spectrum had no significant change in 2 groups within 24 h. The absorbance of UV measured at 284 nm was in line with the pharmacopeia. CONCLUSIONS ：Calcium gluconate injection ， 0.9％ Sodium chloride injection and 5％ Glucose injection have good stability within 24 h，and can be used according to clinical needs.